The GDP-KozakGAL4 insertion line collection consists of a set of stocks in which the coding sequence of a gene of interest has been replaced by a KozakGAL4 promoter trap sequence, resulting in a simultaneous knock-out of the gene of interest and a knock-in of GAL4 driver sequence that is expected to be expressed under the control of the endogenous regulatory sequences of the targeted gene. The 'Associated Files' for this personal communication consist of .xlsx files that were submitted to FlyBase at the time that each set of the fly stocks was sent to the Bloomington Drosophila Stock Center. The .xlsx files contain data primarily concerning the sequence location of the genomic DNA that has been deleted (and replaced with the KozakGAL4 trap sequence), and the identities of the gene(s) that this deletion affects. The gene associations are based on the FlyBase gene annotations at the time of submission of the data.