FB2026_03 , released September 17, 2026
Reference Report
Open Close
Reference
Citation
Ruta, V. (2022.9.16). cADDis sensor constructs and insertions from Vanessa Ruta. 
FlyBase ID
FBrf0254650
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Vanessa Ruta, Rockefeller University.
P{10XUAS-IVS-cADDis(Red-Up)} and PBac{10XUAS-IVS-cADDis(Red-Up)} expresses the cAMP sensor, cADDis (cAMP Difference Detector in situ), under the control of UAS. cADDis is composed of human RAPGEF4 (aka EPAC2) and a circularized red fluorescent protein. Upon cAMP binding, RAPGEF4 changes conformation resulting in an increase in fluorescence levels. The construct was made in pJFRC81 (https://www.addgene.org/36432/). cADDis is described in Tewson et al., 2016, J Biomol Screen 21(3):298-305, PMID: 26657040.
P{10XUAS-IVS-cADDis(Green-Down)} and PBac{10XUAS-IVS-cADDis(Green-Down)} expresses the cAMP sensor, cADDis (cAMP Difference Detector in situ), under the control of UAS. cADDis is composed of human RAPGEF4 (aka EPAC2) and a circularized green fluorescent protein. Upon cAMP binding, RAPGEF4 changes conformation resulting in a decrease in fluorescence levels.  The construct was made in pJFRC81 (https://www.addgene.org/36432/). cADDis is described in Tewson et al., 2016, J Biomol Screen 21(3):298-305, PMID: 26657040.
P{10XUAS-IVS-cADDis(Red-Up)}su(Hw)attP5 is an insertion on the second chromosome into su(Hw)attP5 (FBti0131990).
P{10XUAS-IVS-cADDis(Green-Down)}su(Hw)attP5 is an insertion on the second chromosome into su(Hw)attP5 (FBti0131990).
PBac{10XUAS-IVS-cADDis(Red-Up)}VK00005 is an insertion on the third chromosome into VK00005 (FBti0076428).
PBac{10XUAS-IVS-cADDis(Green-Down)}VK00005 is an insertion on the third chromosome into VK00005 (FBti0076428).
DOI
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference