The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Vanessa Ruta, Rockefeller University.
P{10XUAS-IVS-cADDis(Red-Up)} and PBac{10XUAS-IVS-cADDis(Red-Up)} expresses the cAMP sensor, cADDis (cAMP Difference Detector in situ), under the control of UAS. cADDis is composed of human RAPGEF4 (aka EPAC2) and a circularized red fluorescent protein. Upon cAMP binding, RAPGEF4 changes conformation resulting in an increase in fluorescence levels. The construct was made in pJFRC81 (https://www.addgene.org/36432/). cADDis is described in Tewson et al., 2016, J Biomol Screen 21(3):298-305, PMID: 26657040.
P{10XUAS-IVS-cADDis(Green-Down)} and PBac{10XUAS-IVS-cADDis(Green-Down)} expresses the cAMP sensor, cADDis (cAMP Difference Detector in situ), under the control of UAS. cADDis is composed of human RAPGEF4 (aka EPAC2) and a circularized green fluorescent protein. Upon cAMP binding, RAPGEF4 changes conformation resulting in a decrease in fluorescence levels. The construct was made in pJFRC81 (https://www.addgene.org/36432/). cADDis is described in Tewson et al., 2016, J Biomol Screen 21(3):298-305, PMID: 26657040.
P{10XUAS-IVS-cADDis(Red-Up)}su(Hw)attP5 is an insertion on the second chromosome into su(Hw)attP5 (FBti0131990).
P{10XUAS-IVS-cADDis(Green-Down)}su(Hw)attP5 is an insertion on the second chromosome into su(Hw)attP5 (FBti0131990).
PBac{10XUAS-IVS-cADDis(Red-Up)}VK00005 is an insertion on the third chromosome into VK00005 (FBti0076428).
PBac{10XUAS-IVS-cADDis(Green-Down)}VK00005 is an insertion on the third chromosome into VK00005 (FBti0076428).