FB2026_03 , released September 17, 2026
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Akbari, O. (2022.10.19). P{Hsp70Bb-Cas9.T2A.EGFP.PRE.2gy} construct and insertion from Omar Akbari. 
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FBrf0254784
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Personal communication to FlyBase
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Text of Personal Communication 
The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Omar Akbari, University of California, San Diego.
P{Hsp70Bb-Cas9.T2A.EGFP.PRE.2gy} carries the heat shock promoter from Hsp70Bb regulating expression of a cassette composed of Cas9, followed by the self-cleaving peptide T2A, followed by EGFP.  It also carries Polycomb Response Elements (PREs) and two gypsy insulating elements upstream of the heat shock promoter to reduce leaky expression in the absence of heat shock. For more information, see FBrf0256152.
P{Hsp70Bb-Cas9.T2A.EGFP.PRE.2gy}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
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Related Publication(s)
Research paper

Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible Hsp70Bb promoter in Drosophila melanogaster.
Warsinger-Pepe et al., 2023, G3 (Bethesda) 13(4): jkad024 [FBrf0256152]

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    English
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    Natural transposons (1)
    Insertions (2)
    Transgenic Constructs (1)