Abstract
Translationally controlled tumor protein (TCTP) is a growth and development related protein found in almost all eukaryotes and can be involved in a variety of cellular life activities. Our previous studies found that TCTP is involved in the response of Drosophila Kc cells to deltamethrin (DM) stimulation, and it may be a candidate gene related to DM stress. Therefore, we would further investigate the functions of TCTP and its mechanism under DM stress. The qPCR results showed that the expression level of Drsl1 increasing first and then decreasing with the change of DM concentration and treatment duration. The optimal concentration was 20 ppm and the optimal time was 24 h. qPCR and WB results together showed that the expression levels of TCTP and Drsl1 were positively correlated. The flow cytometry showed the expression levels of TCTP and Drsl1 in deltamethrin-stressed cells are related to apoptosis. The apoptosis rate reached the highest level in the cells with simultaneous interference of both genes. Taken together, our data mainly suggest that TCTP interacts with Drsl1 in response to DM stress in Drosophila Kc cells, which helps to investigate the mechanisms of DM toxicity and the mechanisms by which insects develop resistance to it.
