FB2026_02 , released June 18, 2026
Reference Report
Open Close
Reference
Citation
Rabinow, L. (2023.6.12). P{UAS-Doa.227kD.IR} construct and insertion. 
FlyBase ID
FBrf0256779
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
The following information accompanied stocks donated to the Bloomington Stock Center by Lenny Rabinow, Harvard Medical School.
P{UAS-Doa.227kD.IR} is a pP{UAST}-based transgene expressing dsRNA for RNAi of Doa transcripts RP and RV, which encode the 227 kD Doa isoform. The sequence from Doa used to create the inverted repeat is
TTCTAGAGGGTATATGAGTTCGACTAGAAGTCAGCAATGGAGCTCCCATGGGTTGACCACCACCCGGCACATGCGTCATGGACATGCTCTTTGGAAGCACCTGTTGCTGCTGTGCTGGAAATTGCTGCTTCACAGGCACTCTACTCACAAAATTCGATCCTGCCAACGGCGATTGCTCGTTGCAGATCAACTTATAGAGATCGTCCTTGGTCACCGCCTGACGCACCTTGAGATCCAGGTGCGACAAAGATGCATTTTTCTGCAGTTTGTGGGGCTGATTTTGGGGCACCTGTGACTGCTGAGGTCGCTGCAGCTCCTTCTGAGCGGCCTCCGCAACTGTGGGATCAAATATGAATTC
P{UAS-Doa.227kD.IR}1 is a third chromosome insertion.
Expression of this insertion in the eye suppresses cell death associated with overexpression of the 55 kD, 69 kD, 91 kD, 105 kD and 138 kD Doa isoforms in the eye.
DOI
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Alleles (1)
    Genes (1)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (1)
    Transgenic Constructs (1)