NrkKO is a null allele generated by CRISPR mutagenesis using two guide RNAs within Nrk to delete most coding sequences. One guide RNA targeted sequences within the first exon distal to ND-MWFE sequences. The second guide RNA targeted sequences proximal to the stop codon within exon 4.
The associated file is a schematic of the Nrk gene and environs of the null mutant of Nrk generated via Crispr-Cas9 mediated deletion. Also, below is the sequence of the deleted region.
Sequence deleted in NrkKO
tagtttatgagtgctcgcaaggattttgcttggaaacggttcgctaaacaaggcatatacataaaacacagccatggctgccgggcaatgggtgggggtggttgagcgggtgctccggggaatggtgctgaaatggggggccaatttggctgtcctggggctgtgcgtgtttctctttgccagcgccacgcacgcgaactccctgaacgccatcgaggagcccgtcacccggcgacaccaccagcggcatcacgagcgcgagcgggaggagaacggctactgcgccccgtacagcggcaaggtgtgcaaggaatacctcaccggccaggtgtggtacagtctggaggatcccactggcgggtggaagaacgagcaggtgaccacggcgctctgggacgagcttatctccgatcttacgggtctgtgtcgcgaagcagccgaggtgagtttcgattgatacatctagagatcccatctcatcccattaatctcttttcgctacagaaaatgctctgcgcctatgcgtttcccaactgccacatggagggcggtcgagcggtgaaggctcctctctgcttcgaggattgccaggccacgcatctccagttctgctacaacgactgggtgctcatcgaggagaagaaggagcgaaatatgttcatcaagagccgcggccacttccggctacccaactgctcctccttgccgcactacaacgcttccatgcggcgacccaactgctcctacatcggtctcaccgaactcaaggagtccgaagtgagctgtaagtcttcattatcacaaaccctcaagaaggtctttctgatgctagaatcccttttcttcacagacgattgccgcaatggaaacggacgcttctacatgggcacaatgaacgtgtccaagtcgggcattccctgccagcgctgggacactcagtacccgcacaagcacttccagccaccactggtcttccatcagctcctggagggcgaaaactactgccggaatgctggcggtgaggagccgcatccctggtgctacactgtggatgaatcagtgcgctggcagcactgcgatatacccatgtgtcgtaagatcataaaattttttcctcttttactttttgtaattcacccacttctgttttagcggattatgtggaccccaatgctgtcgatttgaacacgcccatcaagatggagaagttcttcacgccatcgatgatctttctcttggctggaataggtttcgtggccattgtgaccctgcacttgatgatattgctagtctataagttgtccaagcacaaggattactctcagcctgcgggagcagccactgccgaatgcagtgtttccatgcgtggaggaggagattgtggcggcaatctgaacaccagtagagaaaccctcggaggcaatggaaacacgaacaccttggcaaaatggggcaccatcaggagcacggccacaatacacagcaattgcgtggcccttactacggtgaccaatgtgtctgatgcgaagggcacgaaaccgaatgcacgcctggagaagttggagtacccacgcggggatatagtgtatgtgagatcattgggtcaaggagccttcggtcgcgtcttccaggccagggctcctggacttgttcccgatcaggaagatctactagtcgctgttaagatgctaaaggacgacgccagcgaccagatgcagatggatttcgagcgcgaggcctgtttgctggccgagttcgatcatcccaatatcgtgaggctgctgggggtgtgcgccttgggcagacccatgtgcctgctcttcgagtacatggctcctggcgatctaagcgagttcttgcgcgcctgctccccatatgccacacaccaggcgccgacacaggatcgtctgcagttgaacgagctacatctgctgcagatggcggccaacattgcagcgggcatgctgtatctttcggagagaaaattcgtccaccgggatttggccaccaggaattgcctgatcaacgagcacatggcggtaaagatcgccgactttgggctctcgcacaagatctatttgcaggactattacaaaggcgatgagaacgacttcatcccgatccgctggatgccacttgagagcatactgtacaacaagttctcgcttgagtcggatgtgtgggcatacggcatctgtctgtgggaggtcttctccttcgccttgcagccctactttgggttaacccacgagg