Abstract
In vivo imaging of brain activity in Drosophila allows the dissection of numerous types of biologically important neuronal events. A common paradigm involves imaging neuronal Ca[2+] transients, often in response to sensory stimuli. These Ca[2+] transients correlate with neuronal spiking activity, which generates voltage-sensitive Ca[2+] influx. In addition, there is a range of genetically encoded reporters of membrane voltage and of other signaling molecules, such as second-messenger signaling cascade enzymes and neurotransmitters, enabling optical access to a range of cellular processes. Moreover, sophisticated gene expression systems enable access to virtually any single neuron or neuronal group in the fly brain. The in vivo imaging approach enables the study of these processes and how they change during salient sensory-driven events such as olfactory associative learning, when an animal (fly) is presented an odor (a conditioned stimulus) paired with an unconditioned stimulus (an aversive or appetitive stimulus) and forms an associative memory of this pairing. Optical access to neuronal events in the brain allows one to image learning-induced plasticity following the formation of associative memory, dissecting the mechanisms of memory formation, maintenance, and recall.
