The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Elisabeth Knust, Max Planck Institute of Molecular Cell Biology and Genetics.
TI{TI}CdepDelta - CRISPR/Cas9 was used to replace the translation start codon and all coding exons of Cdep with an attP site and 3xP3-DsRed flanked by loxP sites (ordered as follows: attP,loxP,3xP3-DsRed,loxP). The replacement begins 37 bp downstream of the Cdep transcription start site and goes to 8 bp upstream of the 3’UTR. The insertion is homozygous viable and fertile.