FlyBase Reference Report: Schnitzer and Luo, 2025.3.18, M{vas-NLS-TP901-1.nanos3'UTR.RFP-.GFP-} construct and insertion from J. Luo and M.J. Schnitzer.

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Schnitzer, M.J., Luo, J. (2025.3.18). M{vas-NLS-TP901-1.nanos3'UTR.RFP-.GFP-} construct and insertion from J. Luo and M.J. Schnitzer.

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FBrf0261906

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Personal communication to FlyBase

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The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Junjie Luo and Mark Schnitzer, Stanford University.
M{vas-NLS-TP901-1.nanos3'UTR.RFP-.GFP-} carries nuclear-localized Drosophila codon-optimized TP901-1 integrase under the control of vas. It was generated by taking M{vas-NLS-TP901-1.nanos3'UTR.RFP-} and knocking out the GFP marker via CRISPR/Cas9 using PBac{ U6:3-GFP.gRNA.ACMG (FE)}VK00027 as the source of GFP gRNA.
M{vas-NLS-TP901-1.nanos3'UTR.RFP-.GFP-}ZH-2A is a homozygous and hemizygous viable and fertile insertion on the X chromosome into ZH-2A (FBti0076477).

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English

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