Below is the sequencing result of ena1.8 around the site of the deletion.
ena1.8 contains a deletion of approximately 5.3 kB from the gRNA1 binding site to exon 8, and a 305 bp non-coding insertion. 
We were not able to determine the origin of the 305 bp insertion, BLAST within the Drosophila taxid did not find a match for melanogaster. However, the reading frame stops within the insertion and we could not detect protein with antibody staining.
LOCUS       ena_1-8_Sequenci        1292 bp    DNA     linear       14-MAY-2025
FEATURES             Location/Qualifiers
     misc_feature    603..622
                     /note="after deletion"
     misc_feature    278..297
                     /note="before deletion"
     misc_feature    298..602
                     /note="insertion of unknown origin"
     source          1..1292
                     /dnas_title="ena_1-8_Sequencing-consensus"
ORIGIN      
        1 AGCCGAGATC CAAGAGCCTA GGCAAACATA AATACGATAC ATCCGATCCG ATCCGATCGT
       61 TCGGTTTCAT AGTGTGCTTA AGTGTGGGAT CGATCGTGGG ATCTGTTTAG TGTCTGTGAC
      121 GCCTCTGCAA ATATTGGCCA ACGGTTAAtG GCCATGAAGA AGCTCTACGC GAAGACCTCG
      181 TTCACCAGCA AGAAGCCATC GAGTGCCGCC AATTCCACTC CGATATTGGC CTACCACCAG
      241 CAACAGCATC AGCAGCCGGG CAATGGCATT TGCGAGTTCC AGGTGGTGGC ACCTGGTGCC
      301 CACGAATCTG GCCGACGCGG GCAGTTCGTT GTATGAGTGT GAGGCATTTC GCAGGAATGT
      361 TATTTCATCA TTATTTGGCA ATTGCATCAG CCTTTAATTG AGTTGCGATT GGCTGATGGC
      421 ATTTGGTAGT TTTGGAAGGT ACCGTTTTAG CGCGGCGACA GCGCCACAAC GCTGTCGCCG
      481 AGCGTGGTTC GATGGTAGGC ACAAAAGAAG AAGTGTGCGA CGCAAGTCAG TTCAATTCTA
      541 GACTTCGACC ACCGAAACTC GCTGCGCGAT TAGAAAATGG AACAGTTTAA TGACGCGTTC
      601 TCCCGTTTAT TTTTTATTTT TAGGTCACCA CCTCTGCTCC GGAGAATAGC GGCAGCAGCA
      661 CGTCCAGTGG CGGCAGCGGC AACTATGGAA CCATAGGACG AAGCTCTAAT GGCATGGCCT
      721 CCATGATGGA CGAGATGGCC AAGACGCTGG CACGACGACG CGCCCAAGCA GAAAAGAAGG
      781 ATGTAAGTCA ACTTTGTTTA ACTTATTCCA TAATCAATAT CTTAATTCGT TGGCATATTG
      841 CAGCCCGATC CTGAAGCCGA AGTGAAAAAA CGCCCCTGGG AAAAGTCCAA TACACTGCCC
      901 CATAAGCTAA GCGGCGGAGC TGGCAGCGGA TCTGCTGGAA GTGGCCATGA AGGAGCGAAT
      961 GGTAACTCGG GCGGTGCTGG TAGCAACACA ACGAACAGTG GCGGCGAGTC CCCGCGACCC
     1021 ATGCGCAAAC GATTCGGTAG TGCCAGCGAA GAGACCATTC TCAAGGTGGG TGATAACGAG
     1081 ATCATGGTCA CGTTAGAGTT CTGTAACCTT TTATCCCTAA AGCAGGTCAA TGGCGATGGC
     1141 CTGTCGCTGG CGCTTTCCAA TGGCGACTTG GACACACTGA AGGCTGAGAT TGTGCGCGAA
     1201 ATGCGATTGG AGATTCAGAA AGTCAAAAAT GAGATAATAG ATGGTAGGTT GTATAGTATA
     1261 GGCATAGGAA AATTTTCAAT TGACACGGAT TT
//