The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by the GENIE Project, Howard Hughes Medical Institute, Janelia Research Campus. 
P{20XUAS-IVS-Syn21-A1ACR1-EYFP-p10} expresses red-shifted anion channelrhodopsin A1ACR1 codon-optimized for Drosophila and fused at the C-terminal end to EYFP, under UAS control.
P{20XUAS-IVS-Syn21-A1ACR1-EYFP-p10}attP18 is a homozygous and hemizygous viable insertion on the X chromosome into attP18 (FBti0114370).
P{20XUAS-IVS-Syn21-A1ACR1-EYFP-p10}su(Hw)attP1 is a homozygous viable insertion on the third chromosome into su(Hw)attP1 (FBti0131259).
P{13XlexAop2-IVS-Syn21-A1ACR1-EYFP-p10} expresses red-shifted anion channelrhodopsin A1ACR1 codon-optimized for Drosophila and fused at the C-terminal end to EYFP, under lexA control.
P{13XlexAop2-IVS-Syn21-A1ACR1-EYFP-p10}attP18 is a homozygous and hemizygous viable insertion on the X chromosome into attP18 (FBti0114370).
P{13XlexAop2-IVS-Syn21-A1ACR1-EYFP-p10}su(Hw)attP1 is a homozygous viable insertion on the third chromosome into su(Hw)attP1 (FBti0131259).
P{20XUAS-IVS-Syn21-HfACR1-EYFP-p10} expresses red-shifted anion channelrhodopsin HfACR1 codon-optimized for Drosophila and fused at the C-terminal end to EYFP, under UAS control. 
P{20XUAS-IVS-Syn21-HfACR1-EYFP-p10}attP18 is a homozygous and hemizygous viable insertion on the X chromosome into attP18 (FBti0114370).
P{20XUAS-IVS-Syn21-HfACR1-EYFP-p10}su(Hw)attP1 is a homozygous viable insertion on the third chromosome into su(Hw)attP1 (FBti0131259).
P{13XlexAop2-IVS-Syn21-HfACR1-EYFP-p10} expresses red-shifted anion channelrhodopsin HfACR1 codon-optimized for Drosophila and fused at the C-terminal end to EYFP, under lexA control. 
P{13XlexAop2-IVS-Syn21-HfACR1-EYFP-p10}attP18 is a homozygous and hemizygous viable insertion on the X chromosome into attP18 (FBti0114370).
P{13XlexAop2-IVS-Syn21-HfACR1-EYFP-p10}su(Hw)attP1 is a homozygous viable insertion on the X chromosome into attP18 (FBti0114370).
Bushey, D., Shiozaki, H., Shuai, Y., Zheng, J., Jayaraman, V., Hasseman, J.P., Kolb, I., GENIE Project Team, Turner, G.C. (2025). RubyACRs Enable Red-Shifted Optogenetic Inhibition in Freely Behaving Drosophila. bioRxiv. https://doi.org/10.1101/2025.06.13.659144