The following information accompanied stocks donated to the Bloomington Stock Center by Ashish Toshniwal and Jared Rutter, University of Utah.
PBac{UAS-Pcb.T} is a UASt construct expressing wild type Pcb. The Pcb sequences were from the GH06348 cDNA clone using forward primer NotI-ATGTTCATTCCGGCCGCCCAGTCC and reverse primer XhoI-CTATTCGATAATCATAATGAGGTCC.
PBac{UAS-whd.T} is a UASt construct expressing wild type whd. The whd sequences were amplified from the LD31742 cDNA clone using forward primer NotI-ATGGCCGAAGCCCATGCCGCCGTGG and reverse primer XhoI-TTATGTGGATTTGCCGTTTGTGAGTG.
PBac{UAS-Pdk.T} is a UASt construct expressing wild type Pdk. The Pdk sequences were amplified from the LD23669 cDNA clone.
PBac{UAS-LbNOX.T} is a UASt construct expressing Lactobacillus brevis NADH oxidase (coding sequence optimized for human codon usage).
PBac{UAS-mitoLbNOX.T} is a UASt construct expressing Lactobacillus brevis NADH oxidase (coding sequence optimized for human codon usage) with a mitochondrion-targeting tag.
PBac{UAS-Pcb.T}VK00005, PBac{UAS-whd.T}VK00005, PBac{UAS-Pdk.T}VK00005, PBac{UAS-LbNOX.T}VK00005 and PBac{UAS-mitoLbNOX.T}VK00005 are insertions into the PBac{y+-attP-9A}VK00005 target site at 75A10, 3L:17 ,952,108..17,952,108.