The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Xiaona Tang and Carl Wu, Johns Hopkins University.
TI{TI}Trl3xFLAG-mEos3.2 is a homozygous viable and fertile insertion on the third chromosome. It contains a 3xFLAG and mEos3.2 inserted into the N-terminal end of Trl after the ATG, generated by CRISPR/Cas9.
TI{TI}Hsp8324xMS2-V6 is an insertion on the third chromosome. It contains a '24xMS2-V6' stem-loop cassette (see FBrf0261202 for description) inserted into the intron of Hsp83, generated by CRISPR/Cas9.