Abstract
Glucose transporters (GLUTs) are members of the solute carrier (SLC) family 2, which play crucial roles in various physiological processes. In this study, 108 SLC genes were identified from the transcriptomic and Spodoptera litura genome database (ASM270686v3), of which the GLUT genes were the focus. Transcriptome analysis and quantitative Real-time PCR (qPCR) revealed that SlGLUT2 and SlGLUT6 were specifically expressed in Malpighian tubules, and that their expression significantly increased after treatment with various xenobiotics. The structural features of the SlGLUT2 and SlGLUT6 proteins, as well as their glucose transport characteristics, were analyzed using homology modeling and molecular docking. Moreover, the ectopic expression of SlGLUT2 and SlGLUT6 in Drosophila led to a significant increase in the tolerance of the flies to diamide insecticides. Moreover, the knockdown of SlGLUT2 and SlGLUT6 expression through RNA interference (RNAi) decreased the tolerance of S. litura to cyantraniliprole and chlorantraniliprole. Therefore, SlGLUT2 and SlGLUT6 may play important roles in xenobiotic tolerance in S. litura.
