García-Simarro, M.P., Mondéjar-López, M., García-Martínez, J.C., Cuesta-Casas, A., Casas-Tintó, S., Ahrazem, O., Gómez-Gómez, L., Niza, E. (2026). Multifunctional biofunctionalized hybrid nanoantifungals with novel active coating agents based on cinnamaldehyde- and β-cyclocitral-modified polydopamine.  Mater Today Bio 36(): 102645.

FBrf0264271

Research paper

Fungal pathogens pose serious threats to global agriculture, causing substantial crop loss and food security concerns. Current solutions often rely on conventional pesticides, generating negative environmental impacts. This study presents the development of two novel multifunctional hybrid nanoparticles designed to provide a sustainable alternative for crop protection and growth promotion. These nanoparticles are based on dendritic mesoporous silica nanoparticles (dMSNs) and functionalized with innovative coating agents derived from cinnamaldehyde- (CIN) and β-cyclocitral (βETA) -modified polydopamine (DOPA). The purpose of this research was to create a system that could deliver antifungal agents and support plant development simultaneously, especially under pathogen-induced stress. Nanoparticles exhibited a dual-release mechanism with pH-responsive kinetics, releasing up to 94 % of geraniol (GER) and 81 % of compound βETA, at pH 5. This confirms their ability for targeted, stimulus-triggered delivery. In vitro tests showed strong antifungal activity against several plant pathogenic fungi, with minimum inhibitory concentrations down to 0.078 mg/mL. Microscopic analysis revealed significant disruption of fungal mycelia after treatment, confirming the antifungal mechanism. In vivo biosafety was established through assays on Drosophila melanogaster. Furthermore, experiments on plants infected with Fusarium oxysporum demonstrated enhanced seed germination and early plant development. Treated plants showed improved root and shoot growth, higher chlorophyll content, and restored levels of key physiological markers like polyphenols and carotenoids. This study reports two dual-functional nanoparticles that improve the control of fungal pathogens while simultaneously promoting early plant development. The findings highlight their potential as sustainable nanobiotechnological tools for protecting crops and enhancing productivity in agricultural systems affected by fungal diseases.

PMC12775976 (PMC) (EuropePMC)