Abstract
Drosophila melanogaster third-instar larvae were exposed to AlCl3 in the diet for 24 h. Dose-dependent reductions in pupation and adult emergence were observed, Biochemical analyses revealed significantly elevated oxidative stress markers: increased lipid peroxidation and protein oxidation and depletion of reduced glutathione and antioxidant enzymes such as superoxide dismutase, catalase, and glutathione transferase. At higher concentrations, larvae also showed increased β-galactosidase activity and ONPG hydrolysis, suggesting lysosomal dysfunction and senescence-like responses.
