The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Liqun Luo, Stanford University.
TI{TI}FiliFRT(V5).FLAG.RFP+ is similar to TI{TI}FiliFRT(V5).FLAG used in Li et al., 2025, Nature, 649(8097):667, but retains the loxP-flanked mCherry marker. A CRISPR knockin results in conditionally-tagged Fili protein. In the absence of FLP recombinase, Fili is tagged with V5 at the C terminus. In the presence of FLP, V5 is removed and Fili is tagged with FLAG at the C terminus.
TI{TI}kirreFRT(HA).Myc.RFP+ is similar to TI{TI} kirreFRT(HA).Myc used in Li et al., 2025, Nature, 649(8097):667, but retains the loxP-flanked mCherry marker. A CRISPR knockin results in conditionally-tagged kirre protein. In the absence of FLP recombinase, kirre is tagged with HA at the C terminus. In the presence of FLP, HA is removed and kirre is tagged with Myc at the C terminus.
TI{TI}kirreFRT(V5).FLAG.RFP+ is similar to TI{TI} kirreFRT(V5).FLAG used in Li et al., 2025, Nature, 649(8097):667, but retains the loxP-flanked mCherry marker. A CRISPR knockin results in conditionally-tagged kirre protein. A CRISPR knockin results in conditionally-tagged kirre protein. In the absence of FLP recombinase, kirre is tagged with V5 at the C terminus. In the presence of FLP, V5 is removed and kirre is tagged with FLAG at the C terminus.