FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Sequence Feature: Dmel\DRSC00143
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General Information
Symbol
Dmel\DRSC00143
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000000134
Collection
Associated gene
Genomic Location
Chromosome (arm)
2L
Sequence location

2L:2,641,364..2,641,540 [-]

Genomic Maps
Sequence Data
Length
177
Comments

One copy of repeat shown

(Booker, 2006.5)

Sequence

Sequence Downloader
CACACTGTGCCCCATTGTTTGGCCGGGTGTGACTGGCACTCCAAGGCGGTGCCTGCTTGGAACCCACGCCACTTGGCACG
TTCACCTTTGTGCAGCTGGATGCTGTTTGGCAACATCCAGTCGAGCACCCACTTGAAGGTAACTCTGTCGCCCCGCCAGC
TCTCCATTTGCGACCCG
Associated Information
Gene(s) (targeted or local)
    Allele(s)
      Transcripts(s)
        Polypeptide(s)
          Construct
            Experimental Data
            Progenitors
            PCR template

            genomic DNA

            (Booker, 2006.5)
            Primer 1 
            CGGGTCGCAAATGGAGA
            (Booker, 2006.5)
            Primer 2 
            CACACTGTGCCCCATTGTT
            (Booker, 2006.5)
            Comments
            Collection Information
            Collection: DRSC_dsRNA
            Symbol
            DRSC_dsRNA
            Title
            DRSC collection of dsRNA for RNAi in cell culture.
            Source and Progenitors
            Species of derivation
            D. melanogaster
            Strain of derivation
            Vector of progenitor construct
            Description

            Comprehensive collection of double-strand RNA fragments (1-2 dsRNAs per annotated gene), used for genome-wide RNAi-knockdown assays in cell culture.

            Additional data
            Sample preparation
            Collection preparation

            The DRSC RNAi screening library was constructed from genomic DNA amplified using Oregon-R strain. In initial collection, most amplicons were designed to FlyBase rel2 at R.Paro's lab (ZMBH Heidelberg). Primers were selected to target the open reading frames (ORF) of known and predicted genes. Regularly updated to accommodate newly identified genes and new gene models, and to replace amplicons with high potential for off-target effects.

            (Flockhart et al., 2006)
            Mode of assay
            Data analysis
            Cell lines
            Observed in
            Not observed in
            Comments
            Stocks (0)
            External Crossreferences and Linkouts ( 1 )
            Crossreferences
            DRSC - Results frm RNAi screens
            Synonyms and Secondary IDs (2)
            Reported As
            Symbol Synonym
            DRSC00143
            HDC00431
            (Booker, 2006.5)
            Secondary FlyBase IDs
            • FBri0000134
            References (1)