FB2026_03 , released September 17, 2026
Sequence Feature: Dmel\dsRNA-GD5973
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General Information
Symbol
Dmel\dsRNA-GD5973
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000075582
Collection
Associated gene
Genomic Location
Chromosome (arm)
3L
Sequence location

3L:1,112,018..1,112,328 [-]

Genomic Maps
Sequence Data
Length
311
Comments

One copy of repeat shown

(Dietzl et al., 2007)

Sequence

Sequence Downloader
TTGCTGCCTGGATGCCTGCTGGTTGCAGGTCGGCGGCGGTGCACGTTTCTCGGAGGAGAGGCACCGCAGTCGGCGATTCT
GATTCCGACGCTACCGCAGACGCGTCGTGTTTCTTCGTGGGCGATGCGGCAGAGTCGAAAGACAACTATTTTGGCTGCTA
CTGTGCCAGGTTGCAACTGCAGCAGCAGCCGCAGCAGCAACATCAGCGTGCCCAACGATGCATGCAAAACAGTCGCTGTT
GTTGCTGCTGTTGCTGATGTTGCCGCAAATGATGGCTACAACTTCTTCAGCATCCGAAGACGAGGATGTGG
Associated Information
Gene(s) (targeted or local)
    Allele(s)
      Transcripts(s)
        Polypeptide(s)
          Construct
          Experimental Data
          Progenitors
          PCR template

          genomic DNA

          (Dietzl et al., 2007)
          Primer 1 
          TTGCTGCCTGGATGCCTGCT
          (Dietzl et al., 2007)
          Primer 2 
          CCACATCCTCGTCTTCGGATGCT
          (Dietzl et al., 2007)
          Comments
          Collection Information
          Collection: VDRC-GD
          Symbol
          VDRC-GD
          Title
          A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC first generation.
          Source and Progenitors
          Species of derivation
          D. melanogaster
          Strain of derivation
          Vector of progenitor construct
          pP{MF3}
          Description

          Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.

          (Dietzl et al., 2007)

          NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

          (FlyBase Curators, 2013)
          Additional data
          Sample preparation
          Collection preparation

          A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.

          (Dietzl et al., 2007)
          Mode of assay
          Data analysis

          A specificity score (Q) was calculated for each construct, such that if Q is < 0.5, off-target effects are probable and if Q > 0.8, off-target effects are improbable.

          (Dickson et al., 2007.7.18)
          Cell lines
          Observed in
          Not observed in
          Comments

          Targeted gene annotation CG32334 was removed in subsequent FlyBase update.

          (dos Santos, 2023.2.8)
          Stocks (0)
          External Crossreferences and Linkouts ( 0 )
          Synonyms and Secondary IDs (2)
          Reported As
          Symbol Synonym
          5973
          (Dietzl et al., 2007)
          dsRNA-GD5973
          Secondary FlyBase IDs
          • FBri0072793
          References (2)