FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Sequence Feature: Dmel\dsRNA-GD10303
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General Information
Symbol
Dmel\dsRNA-GD10303
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000083284
Collection
Associated gene
Genomic Location
Chromosome (arm)
2L
Sequence location

2L:21,422,057..21,422,378 [+]

2L:21,451,254..21,451,575 [+]

2L:21,461,375..21,461,696 [+]

2L:21,456,315..21,456,636 [+]

2L:21,471,461..21,471,782 [+]

2L:21,476,504..21,476,825 [+]

2L:21,481,547..21,481,868 [+]

2L:21,466,418..21,466,739 [+]

2L:21,437,213..21,437,534 [+]

Genomic Maps
Sequence Data
Length
322
Comments

One copy of repeat shown

(Dietzl et al., 2007)

Sequence

Sequence Downloader
CCCCACCAGCGACAGTTGAGAAGAAAGTGGTCCAAAAAAAGGCATCTGGATCTGCTGGCACAAAGGCAAAGAAAGCCTCT
GCGACGCCGTCACATCCGCCAACTCAGCAAATGGTGGACGCTTCCATTAAAAATTTAAAGGAACGTGGCGGTTCATCACT
TCTGGCAATCAAAAAATATATCACTGCCACTTATAAATGCGACGCCCAAAAGTTAGCGCCATTCATCAAGAAGTACTTAA
AATCGGCCGTGGTCAATGGAAAGCTTATCCAAACTAAGGGAAAGGGTGCATCTGGATCTTTCAAACTGTCGGCCTCTGCC
AA
Associated Information
Experimental Data
Progenitors
PCR template

genomic DNA

(Dietzl et al., 2007)
Primer 1 
CCCCACCAGCGACAGTTGAGA
(Dietzl et al., 2007)
Primer 2 
TTGGCAGAGGCCGACAGTTTG
(Dietzl et al., 2007)
Comments
Collection Information
Collection: VDRC-GD
Symbol
VDRC-GD
Title
A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC first generation.
Source and Progenitors
Species of derivation
D. melanogaster
Strain of derivation
Vector of progenitor construct
pP{MF3}
Description

Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.

(Dietzl et al., 2007)

NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

(FlyBase Curators, 2013)
Additional data
Sample preparation
Collection preparation

A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.

(Dietzl et al., 2007)
Mode of assay
Data analysis

A specificity score (Q) was calculated for each construct, such that if Q is < 0.5, off-target effects are probable and if Q > 0.8, off-target effects are improbable.

(Dickson et al., 2007.7.18)
Cell lines
Observed in
Not observed in
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
10303
(Dietzl et al., 2007)
dsRNA-GD10303
Secondary FlyBase IDs
  • FBri0076724
References (2)