FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Sequence Feature: Dmel\Insulator_I_28
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General Information
Symbol
Dmel\Insulator_I_28
Species
D. melanogaster
Feature type
insulator
FlyBase ID
FBsf0000154991
Collection
Associated gene
Genomic Location
Chromosome (arm)
2L
Sequence location

2L:347,279..347,289

Genomic Maps
Sequence Data
Length
Comments
Associated Information
Gene(s) (targeted or local)
    Allele(s)
      Transcripts(s)
        Polypeptide(s)
          Construct
            Experimental Data
            Binding data
            Bound moiety
            Cp190 protein
            (Negre et al., 2010.2.22)
            BEAF-32 protein
            (Negre et al., 2010.2.22)
            CTCF protein
            (Negre et al., 2010.2.22)
            Comments on moiety

            Insulator I site defined as binding site for at least 2 components among bound moieties.

            (Negre et al., 2010.2.22)
            Mapped to restriction fragment
            Evidence or Assay
            Collection Information
            Collection: Insulator_Class_I.mE01
            Symbol
            Insulator_Class_I.mE01
            Title
            Insulators (type I) identified by ChIP-chip of Cp190, BEAF-32 and CTCF in embryos.
            Source and Progenitors
            Species of derivation
            D. melanogaster
            Strain of derivation
            Stage
            Tissue/Position (including subcellular localization)
            Reference
            embryonic stage 1 -- 15
            organism

            Comment: 0-12 hr AEL

            (Negre et al., 2010.2.22)
            Vector of progenitor construct
            Description

            Genomic sequences identified as probable insulator elements: chromatin immunoprecipitation using antibody against putative insulator-binding proteins; DNA sequences identifed by hybridization to genomic tiling array. Class I insulator elements were computed as binding sites at 1% FDR for at least 2 components among CP190 (Cp190), BEAF32 (BEAF-32) and CTCF (CTCF).

            (Negre et al., 2010.2.22)
            Additional data
            Sample preparation
            Collection preparation
            Mode of assay
            Data analysis

            Insulator binding data was processed with Model based Analysis Tiling-arrays (MAT) software (Johnson et al., 2006, Proc Natl Acad Sci USA 103:12457-12462 ). We ran paired MAT analysis with MaxGap of 500, MinProbe of 10, and a Bandwidth of 250. We report here the peaks found by MAT at 1% FDR. Class I insulator elements were computed as binding sites at 1% FDR for at least 2 components among CP190, BEAF32 and CTCF, with overlap by less than 250bp between their peak center (mid-peak) location.

            (Nègre et al., 2010)
            Cell lines
            Observed in
            Not observed in
            Comments
            Stocks (0)
            External Crossreferences and Linkouts ( 0 )
            Synonyms and Secondary IDs (1)
            Reported As
            Symbol Synonym
            Insulator_I_28
            Secondary FlyBase IDs
              References (1)