FB2026_03 , released September 17, 2026
Sequence Feature: Dmel\dsRNA-4989R
Open Close
General Information
Symbol
Dmel\dsRNA-4989R
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000432773
Collection
Associated gene
Genomic Location
Chromosome (arm)
2R
Sequence location

2R:17,733,661..17,734,031 [+]

Genomic Maps
Sequence Data
Length
371
Comments

One copy of repeat shown.

(Japanese National Institute of Genetics, 2012.5.21)

Sequence

Sequence Downloader
ATGCCCAGAGGAACTGGGATATATTCTACAAGCGAAATGAGACTCGTTTCTTTAAGGACCGCCACTGGACAACGCGTGAG
TTCCAGGAGCTGCTGGACCAGGAGGAGTTCCACGAGAAACGAACACTTTTCGAAGTGGGATGCGGTGTGGGCAACCTGGT
ATTCCCGCTGCTGGAAGAACAAACCAGCGAAGAGGGATGTTTCAGCAATAGCAGATTCTTCTTCTACGCGTGCGATTTCT
CACCGCGAGCAGTGGAATTCGTGCGCTCCAATCCTCTGTACGATCCCAGCCAGATATCTGCCTTTCAGTGTGATATCACA
ACGCAACAGGTGCATGACCACATCCCTCCCAGCAGCGTGGACATTTGCACG
Associated Information
Gene(s) (targeted or local)
Allele(s)
Transcripts(s)
    Polypeptide(s)
      Construct
      Experimental Data
      Progenitors
      PCR template

      cDNA

      (Japanese National Institute of Genetics, 2012.5.21)
      Primer 1 
      aaggcctacatggccggaccgATGCCCAGAGGAACTGGGATA
      (Japanese National Institute of Genetics, 2012.5.21)
      Primer 2 
      aatctagaggtaccCGTGCAAATGTCCACGCTGCT
      (Japanese National Institute of Genetics, 2012.5.21)
      Comments
      Collection Information
      Collection: NIG_RNAi_Fly-1
      Symbol
      NIG_RNAi_Fly-1
      Title
      A set of transgenic RNAi constructs for expression of dsRNA under UAS control, NIG-FLY.
      Source and Progenitors
      Species of derivation
      D. melanogaster
      Strain of derivation
      Vector of progenitor construct
      Description

      Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.

      (Japanese National Institute of Genetics, 2012.5.21)

      NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

      (FlyBase Curators, 2013)
      Additional data
      Sample preparation
      Collection preparation

      A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.

      (Japanese National Institute of Genetics, 2012.5.21)
      Mode of assay
      Data analysis
      Cell lines
      Observed in
      Not observed in
      Comments

      Targeted gene annotation CG4989 was removed in subsequent FlyBase update.

      (dos Santos, 2023.2.8)
      Stocks (1)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      4989R
      (Japanese National Institute of Genetics, 2012.5.21)
      dsRNA-4989R
      Secondary FlyBase IDs
        References (2)