FB2026_03 , released September 17, 2026
Sequence Feature: Dmel\dsRNA-5458R
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General Information
Symbol
Dmel\dsRNA-5458R
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000432999
Collection
Associated gene
Genomic Location
Chromosome (arm)
2L
Sequence location

2L:13,184,096..13,184,115 [+]

2L:13,183,650..13,183,663 [+]

2L:13,183,828..13,183,838 [+]

2L:13,183,839..13,183,895 [+]

2L:13,183,896..13,183,943 [+]

2L:13,183,944..13,183,951 [+]

2L:13,183,952..13,183,993 [+]

2L:13,183,994..13,184,003 [+]

2L:13,184,004..13,184,038 [+]

2L:13,184,039..13,184,045 [+]

2L:13,184,046..13,184,069 [+]

2L:13,184,070..13,184,095 [+]

2L:13,184,116..13,184,313 [+]

Genomic Maps
Sequence Data
Length
511
Comments

One copy of repeat shown.

(Japanese National Institute of Genetics, 2012.5.21)

Sequence

Sequence Downloader
GACCCAATATAGGCCTCTACATAGGCCGGTCGAAATGCTGCCGGACAGAGACAAGGTAGAGGTTGGGCCATCCTGCCCAA
CGGTAGATCAGTATGATGGGAACTACAGGAAGGGTCGCCGTCACGGCATCGGTGGTGTACGATGTTCAAGGATGGTTCAC
GATACTACGGACAGTATCGCTGCGAGGAAGCGTTGTTGGGCGCGGCATCTTCATCTATCCGGACGGATCCGATGTACGAC
GGGCAACTGGCGAAAGAACTTGAATGCNCGGCAAGGGCCGGTACAAGTATGGTGAATGGGGACAACTATTCGTGGTGACT
GGTTCAAGGGACAACGTCATGGGGTGGGCATCTATCACTTTAACAGCGGAAAGGATgGCTGCTGCCTGTCCGTCAGAATG
AAGGCCACCTGGAACAGCAACATGCGAACGGGTCCCTTCGAGCTGTATATCGGCAATGAGGACAAGTGCACAATACTTCA
CGGAATTTGGGATAACCTGTACCCGAGTGGA
Associated Information
Gene(s) (targeted or local)
Allele(s)
Transcripts(s)
    Polypeptide(s)
      Construct
      Experimental Data
      Progenitors
      PCR template

      cDNA

      (Japanese National Institute of Genetics, 2012.5.21)
      Primer 1 
      aaggcctacatggccggaccgGACCCAATATAGGCCTCTACA
      (Japanese National Institute of Genetics, 2012.5.21)
      Primer 2 
      aatctagaggtaccTCCACTCGGGTACAGGTTATC
      (Japanese National Institute of Genetics, 2012.5.21)
      Comments
      Collection Information
      Collection: NIG_RNAi_Fly-1
      Symbol
      NIG_RNAi_Fly-1
      Title
      A set of transgenic RNAi constructs for expression of dsRNA under UAS control, NIG-FLY.
      Source and Progenitors
      Species of derivation
      D. melanogaster
      Strain of derivation
      Vector of progenitor construct
      Description

      Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.

      (Japanese National Institute of Genetics, 2012.5.21)

      NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

      (FlyBase Curators, 2013)
      Additional data
      Sample preparation
      Collection preparation

      A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.

      (Japanese National Institute of Genetics, 2012.5.21)
      Mode of assay
      Data analysis
      Cell lines
      Observed in
      Not observed in
      Comments
      Stocks (0)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      5458R
      (Japanese National Institute of Genetics, 2012.5.21)
      dsRNA-5458R
      Secondary FlyBase IDs
        References (1)