FB2026_03 , released September 17, 2026
Sequence Feature: Dmel\dsRNA-8253Rb
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General Information
Symbol
Dmel\dsRNA-8253Rb
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000434269
Collection
Associated gene
Genomic Location
Chromosome (arm)
2R
Sequence location

2R:5,665,405..5,665,786 [+]

Genomic Maps
Sequence Data
Length
382
Comments

One copy of repeat shown.

(Japanese National Institute of Genetics, 2012.5.21)

Sequence

Sequence Downloader
CAACGATCATATTCGTGCGCTAAATAAAATGGGCTCAACGGAACAAATCTCCGGATGTACAAGTACTTGTTCAAATGCTG
GATCCGGCGAGTCAGGCGAAATGGGAAGAGCTTTTAGAGGACCCAGTGTTCAAAAATGCAATTTCGACGTGGGAATCCAT
GGCATTGCGTCTGGACCAGCAGTAAAAGACGCTGGAGAACATGGAGTTCGCCATGGCAAGCTTAACGCTTGTAATAGAAC
ACCTGCTTTGTGTAGGTCCGACCTTCTTGCCACAAGCCTCAGCCCTGCGACCTGTGCCTTCTGTCATGCTTCTGGTCACT
CAATTTATCACTTCCAAAGTTTTAAAAGCCTTAACCAGCAAGTCGATTGCAAGAAGCGAAGA
Associated Information
Gene(s) (targeted or local)
    Allele(s)
      Transcripts(s)
        Polypeptide(s)
          Construct
          Experimental Data
          Progenitors
          PCR template

          cDNA

          (Japanese National Institute of Genetics, 2012.5.21)
          Primer 1 
          aaggcctacatggccggaccgCAACGATCATATTCGTGCGCT
          (Japanese National Institute of Genetics, 2012.5.21)
          Primer 2 
          aatctagaggtaccTCTTCGCTTCTTGCAATCGAC
          (Japanese National Institute of Genetics, 2012.5.21)
          Comments
          Collection Information
          Collection: NIG_RNAi_Fly-1
          Symbol
          NIG_RNAi_Fly-1
          Title
          A set of transgenic RNAi constructs for expression of dsRNA under UAS control, NIG-FLY.
          Source and Progenitors
          Species of derivation
          D. melanogaster
          Strain of derivation
          Vector of progenitor construct
          Description

          Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.

          (Japanese National Institute of Genetics, 2012.5.21)

          NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

          (FlyBase Curators, 2013)
          Additional data
          Sample preparation
          Collection preparation

          A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.

          (Japanese National Institute of Genetics, 2012.5.21)
          Mode of assay
          Data analysis
          Cell lines
          Observed in
          Not observed in
          Comments

          Targeted gene annotation CG1294 was removed in subsequent FlyBase update.

          (dos Santos, 2023.2.8)
          Stocks (0)
          External Crossreferences and Linkouts ( 0 )
          Synonyms and Secondary IDs (2)
          Reported As
          Symbol Synonym
          8253Rb
          (Japanese National Institute of Genetics, 2012.5.21)
          dsRNA-8253Rb
          Secondary FlyBase IDs
            References (2)