FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Sequence Feature: Dmel\ChIP-chip_bap_E6-8h_000123
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General Information
Symbol
Dmel\ChIP-chip_bap_E6-8h_000123
Species
D. melanogaster
Feature type
TF_binding_site
FlyBase ID
FBsf0000542184
Collection
Associated gene
Genomic Location
Chromosome (arm)
2L
Sequence location

2L:7,507,722..7,507,922

Genomic Maps
Sequence Data
Length
Comments
Associated Information
Gene(s) (targeted or local)
    Allele(s)
      Transcripts(s)
        Polypeptide(s)
          Construct
            Experimental Data
            Binding data
            Bound moiety
            bap protein
            (Monfort and Furlong, 2015.1.15)
            Comments on moiety
            Mapped to restriction fragment
            Evidence or Assay
            Collection Information
            Collection: ChIP-chip_bap_E6-8h_organism
            Symbol
            ChIP-chip_bap_E6-8h_organism
            Title
            ChIP-chip peak calls for bap from D. melanogaster, embryo (6-8 hr AEL).
            Source and Progenitors
            Species of derivation
            D. melanogaster
            Strain of derivation
            Stage
            Tissue/Position (including subcellular localization)
            Reference
            embryonic stage 10 -- 11
            organism

            Comment: 6-8 hr AEL

            (Zinzen et al., 2009)
            Vector of progenitor construct
            Description
            Additional data
            Sample preparation
            Collection preparation

            Two independent chromatin immunoprecipitations (ChIPs) were performed using two different anti-bap antibodies (FBrf0131347, FBrf0201841). These ChIPs were compared to two independent mock ChIPs using rabbit pre-immune sera, as previously described (FBrf0202481).

            Chromatin was prepared from formaldehyde-fixed embryos as previously described (FBrf0202481).

            Mode of assay

            Immunoprecipitated chromatin was analyzed by Affymetrix GeneChip Drosophila Tiling 1.0R array

            Data analysis

            Peaks were called with TileMap software (v1) after quantile normalization. For each enriched region, probe intensity peaks were identified as extrema on a smoothed curve of the log2-ratio signal. Finally, 200 bp regions centered on identified extremas were defined. Peak calls were lifted over from Dmel_Release_5 to Dmel_Release_6 using the NCBI Genome Remapping tool.

            Cell lines
            Observed in
            Not observed in
            Comments

            log2ratio_IP_over_input=1.460

            (Monfort and Furlong, 2015.1.15)
            Stocks (0)
            External Crossreferences and Linkouts ( 0 )
            Synonyms and Secondary IDs (2)
            Reported As
            Symbol Synonym
            ChIP-chip_bap_E6-8h_000123
            bap_6-8h_123
            (Monfort and Furlong, 2015.1.15)
            Secondary FlyBase IDs
              References (1)