FB2026_02 , released June 18, 2026
Sequence Feature: Dmel\sgRNA-GS05214
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General Information
Symbol
Dmel\sgRNA-GS05214
Species
D. melanogaster
Feature type
sgRNA
FlyBase ID
FBsf0000764623
Collection
Associated gene
Genomic Location
Chromosome (arm)
2L
Sequence location

2L:19,484,574..19,484,596 [+]

Genomic Maps
Sequence Data
Length
23
Comments

Sequence

Sequence Downloader
ACTTGGTAGGAGATGCAGGTTGG
Associated Information
Gene(s) (targeted or local)
Allele(s)
Transcripts(s)
    Polypeptide(s)
      Construct
      Experimental Data
      Collection Information
      Collection: TRiP-KO
      Symbol
      TRiP-KO
      Title
      A set of transgenic constructs for sgRNA-targeted gene mutation.
      Source and Progenitors
      Species of derivation
      D. melanogaster
      Strain of derivation
      Vector of progenitor construct
      Description

      A set of transgenic constructs that each consist of a short guide RNA (sgRNA) targeting the coding sequence of a gene. The sgRNA is under the control of a U6:3 (ubiquitous) promoter. When crossed to a stock that contains a source of Cas9, cleavage and mutagenesis of the targeted gene is induced, resulting in a small deletion or insertion mutation.

      (Transgenic RNAi Project members, 2017-)
      Additional data
      Sample preparation
      Collection preparation

      pCFD3 and pCFD4 vectors described at [CRISPR fly design](http://www.crisprflydesign.org/plasmids/)

      (Transgenic RNAi Project members, 2017-)
      Mode of assay
      Data analysis
      Cell lines
      Observed in
      Not observed in
      Comments
      Stocks (1)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      sgRNA-GS05214
      Secondary FlyBase IDs
        References (1)