FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Sequence Feature: Dmel\sgRNA-GS05200.1
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General Information
Symbol
Dmel\sgRNA-GS05200.1
Species
D. melanogaster
Feature type
sgRNA
FlyBase ID
FBsf0000764637
Collection
Associated gene
Genomic Location
Chromosome (arm)
3R
Sequence location

3R:8,723,277..8,723,299 [+]

Genomic Maps
Sequence Data
Length
23
Comments

Sequence

Sequence Downloader
CAGGCTGTGCCCCAAAGCCGTGG
Associated Information
Gene(s) (targeted or local)
Allele(s)
    Transcripts(s)
      Polypeptide(s)
        Construct
          Experimental Data
          Collection Information
          Collection: TRiP-KO
          Symbol
          TRiP-KO
          Title
          A set of transgenic constructs for sgRNA-targeted gene mutation.
          Source and Progenitors
          Species of derivation
          D. melanogaster
          Strain of derivation
          Vector of progenitor construct
          Description

          A set of transgenic constructs that each consist of a short guide RNA (sgRNA) targeting the coding sequence of a gene. The sgRNA is under the control of a U6:3 (ubiquitous) promoter. When crossed to a stock that contains a source of Cas9, cleavage and mutagenesis of the targeted gene is induced, resulting in a small deletion or insertion mutation.

          (Transgenic RNAi Project members, 2017-)
          Additional data
          Sample preparation
          Collection preparation

          pCFD3 and pCFD4 vectors described at [CRISPR fly design](http://www.crisprflydesign.org/plasmids/)

          (Transgenic RNAi Project members, 2017-)
          Mode of assay
          Data analysis
          Cell lines
          Observed in
          Not observed in
          Comments
          Stocks (1)
          External Crossreferences and Linkouts ( 0 )
          Synonyms and Secondary IDs (1)
          Reported As
          Symbol Synonym
          sgRNA-GS05200.1
          Secondary FlyBase IDs
            References (1)