FB2026_03 , released September 17, 2026
Sequence Feature: Dmel\sgRNA-GS03199
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General Information
Symbol
Dmel\sgRNA-GS03199
Species
D. melanogaster
Feature type
sgRNA
FlyBase ID
FBsf0000910588
Collection
Associated gene
Genomic Location
Chromosome (arm)
3L
Sequence location

3L:5,845,709..5,845,731 [+]

Genomic Maps
Sequence Data
Length
23
Comments

Sequence

Sequence Downloader
AGCATTCACAACGTATCTCACGG
Associated Information
Gene(s) (targeted or local)
Allele(s)
Transcripts(s)
    Polypeptide(s)
      Construct
      Experimental Data
      Collection Information
      Collection: TRiP-OE-flySAM
      Symbol
      TRiP-OE-flySAM
      Title
      A set of transgenic sgRNA constructs for targeted gene overexpression by flySAM.
      Source and Progenitors
      Species of derivation
      D. melanogaster
      Strain of derivation
      Vector of progenitor construct
      Description

      A set of transgenic constructs that each consist of single sgRNA that contains MS2 loops. The tagged sgRNA forms one component of the three-component flySAM (synergistic activation mediator) system, which is designed to produce effective transcriptional activation of the gene targeted by the sgRNA. When crossed to a stock containing the other two components of the flySAM system (a source of nuclease-dead Cas9 fused to a transcriptional activation domain plus an MCP-tagged transcriptional activator), the targeted gene will be overexpressed wherever the three components are expressed together.

      (Transgenic RNAi Project members, 2017-)
      Additional data
      Sample preparation
      Collection preparation
      Mode of assay
      Data analysis
      Cell lines
      Observed in
      Not observed in
      Comments
      Stocks (0)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      sgRNA-GS03199
      Secondary FlyBase IDs
        References (1)