This is a clonal derivative of Kc167-IPPI-66D (described in FBrf0223589), with an mCherry coding sequence driven by a MtnA promoter plus a methotrexate-resistance marker inserted between the attP sites of the docking platform, catalyzed by phiC31:int integrase.

This line contains a single copy of the Mt-mCherry transcription unit, contained in an insulated docking site at polytene position 66D. Compared with Mt-mCherry lines made by more traditional stable transformation procedures, this line has a lower basal (without Cu treatment) expression, a higher induction ratio with Cu, and much less cell-to-cell variation in mCherry expression.