FlyBase Insertion Report: Dmel\PBac{PB}CG3335[c05958]

General Information

Symbol

Dmel\PBac{PB}CG3335^c05958

Species

D. melanogaster

Name

FlyBase ID

FBti0041260

Feature type

transposable_element_insertion_site

Description

Inserted Element

PBac{PB}

LINE ID

c05958

Affected gene(s)

CG3335

Viability / fertility

Associated allele(s)

CG3335^c05958

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 67C2 )

Sequence Location

3L:9,625,150..9,625,150 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_PBac{PB}.Exel.c

Description

PBac{PB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.

(Bellen et al., 2011)

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{PB}. The PBac{PB} construct carries the w^+mC mini-white marker flanked by short (48-bp) Scer\FRT recombination sites.

(Thibault et al., 2004)

Detailed Mapping Data

Chromosome (arm)

(Thibault, 2004, Thibault, 2004)

Sequence Location

3L:9,625,150..9,625,150 [-]

(Levis, 2009.11.17)

Orientation

(Levis, 2018.1.2, Levis, 2009.11.17, Thibault, 2004, Thibault, 2004)

Cytological location (computed by FlyBase)

67C2 (inferred by FlyBase from sequence location)

Cytological location (reported)

67C2_r6 (reported as inferred from sequence location)

(Levis, 2018.1.2)

Insertion into Natural transposon

Comments concerning location

Location is approximate. Sequence does not extend to vector-flank junction, but is probably within 20 bp.

(Levis, 2009.11.17)

The insertion site location is not precise because the readable 5' flank sequence does not extend to the vector-flank junction, but is probably < 20 bp to the left of position 9625150 (release 6).

(Levis, 2018.1.2)

Sequence Data

Flanking sequence

KS718213

(Levis, 2018.1.2)

Inserted Element

Construct

PBac{PB}

Size (Kb)

7.257

Component Allele(s)

w^+mPB.FRT