PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
The location is not precise since the readable 5' flanking sequence ends at an undetermined distance from the vector-flank junction (probably < 200 bp). The flanking sequence aligns well to two sites in a local duplication at X:7897371 in the CR11386 gene and X:7898561 in the CR33222 gene.
f07325