FlyBase Insertion Report: Dmel\PBac{WH}cv-c[f07633]

General Information

Symbol

Dmel\PBac{WH}cv-c^f07633

Species

D. melanogaster

Name

FlyBase ID

FBti0042681

Feature type

transposable_element_insertion_site

Description

Inserted Element

PBac{WH}

LINE ID

f07633

Affected gene(s)

cv-c

Viability / fertility

Associated allele(s)

cv-c^f07633

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 88B8_r6 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_PBac{WH}.Exel.f

Description

PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.

(Bellen et al., 2011)

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w^+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.

(Thibault et al., 2004)

Detailed Mapping Data

Chromosome (arm)

(Thibault, 2004, Thibault, 2004)

Sequence Location

Orientation

(Levis, 2018.1.2, Thibault, 2004, Thibault, 2004)

Cytological location (computed by FlyBase)

Cytological location (reported)

88B8_r6 (reported as inferred from sequence location)

(Levis, 2018.1.2)

Insertion into Natural transposon

Comments concerning location

The insertion site location is not precise because the readable 3' flank sequence does not extend to the vector-flank junction. The actual insertion site location is to the right of position 14399430 (release 6), probably < 100 bp away.

(Levis, 2018.1.2)

Sequence Data

Flanking sequence

KS718243

(Levis, 2018.1.2)

Inserted Element

Construct

PBac{WH}

Size (Kb)

7.234

Component Allele(s)

w^+mC