PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
e00855
Exelixis, Inc. determined the insertion site of PBac{RB}e00855 to be at Release 3 genomic coordinate 5066024 on chromosome arm 2L.The Gene Disruption project determined the insertion site of PBac{RB}e00855 to be at Release 3 genomic coordinate 5066024 on arm 2L.