PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
3L
Exelixis, Inc. determined the insertion site of PBac{RB}CG8001e01500 to be at Release 3 genomic coordinate 1737276 on chromosome arm 3L.The Gene Disruption Project determined the insertion site of PBac{RB}CG8001e01500 to be at Release 3 genomic coordinate 1737279 on arm 3L.
Location 3L:1740181-1740182 confirmed by FlyBase alignment of dbGSS accessions CZ479316 and CZ479315 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.