PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
Location 3R:25693610-25693611 confirmed by FlyBase alignment of dbGSS accession CZ482778 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
Accession CZ482778 more definitive. Accession CZ482779 is comprised mostly of S-element sequences, but 326-415 aligns to a unique site in CG7816, 17bp from site defined by CZ482778; since CZ482779 appears not to extend to the insertion site, location defined by CZ482778 likely to be correct.