PpyrLUCrgr-16 is seen to express in a set of neurons in the anterior medial cortex of the larval protocerebral lobe. These neurons extend neurites to the contralateral lobe. Strong expression is seen in a pair mesothoracic neurons, and many lateral segmentally reiterated cells of the larval ventral nerve cord. In adults, expression of PpyrLUCrgr-16 is seen in cells found close to the lobulla and medulla of the optic lobes, and in cells that both contribute to the superior arch commissure and innervate the superior medial protocerebrum