Additional lines created; use of a high-throughput embryo sorter allowed screening of very large numbers of animals for GFP-expressing protein trap insertions.
A set of transgenic insertion stocks derived by TE mobilization using the protein-trap constructs P{PTT-GA}, P{PTT-GB}, and P{PTT-GC}. The constructs carry a w+mC mini-white visible marker and an Avic\GFP vital fluorescent protein-trap marker. In each of the three constructs, the splice acceptor and splice donor consensus sequences are in a different reading frame relative to the Avic\GFP sequence. For a successful GFP-positive insertion into an intron of a protein-coding gene, translation results in a fusion of the GFP to both the amino- and carboxyl-terminal parts of the trapped protein.
The GFP-tagged M6 is detected in the follicular epithelium throughout oogenesis. It is strongly expressed in anterior and posterior polar follicle cells and in migrating border cells. It is absent from the the squamous follicle cells overlying the nurse cells in stages S9-12 and absent from the germline.
Protein trap line.