The attP cassette of the Mi{MIC} element has been replaced with the attB protein-trap cassette from the pBS-KS-attB1-2-PT-SA-SD-1-EGFP-FlAsH-StrepII-TEV-3xFlag plasmid by phiC31-mediated recombination. The attB protein-trap cassette contains a multi-tag cassette consisting of the EGFP version of Avic\GFP, T:Zzzz\TC, T:Zzzz\StrepII, T:Zzzz\TEV.CS and 3xT:Zzzz\FLAG.
The attP cassette of the Mi{MIC} element has been replaced with the attB protein-trap cassette from the pBS-KS-attB1-2-PT-SA-SD-1-EGFP-FlAsH-StrepII-TEV-3xFlag plasmid by phiC31-mediated recombination. The attB protein-trap cassette contains a multi-tag cassette consisting of the EGFP version of Avic\GFP, T:Zzzz\TC, T:Zzzz\StrepII, T:Zzzz\TEV.CS and 3xT:Zzzz\FLAG.