FlyBase Insertion Report: Dmel\Mi{Trojan-QF2.2}VGlut1[MI04979-TQF2.2]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Insertion: Dmel\Mi{Trojan-QF2.2}VGlut1^{MI04979-TQF2.2}

General Information

Symbol

Dmel\Mi{Trojan-QF2.2}VGlut1^{MI04979-TQF2.2}

Species

D. melanogaster

Name

FlyBase ID

FBti0168166

Feature type

transposable_element_insertion_site

Also Known As

VGlut^MI04979-QF2, VGlut-QF, Vglut-QF2

Description

Inserted Element

Mi{Trojan-QF2.2}

LINE ID

Affected gene(s)

Viability / fertility

Associated allele(s)

Ncra\QF^{VGlut1-MI04979-TQF2.2}, VGlut1^{MI04979-TQF2.2}

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 22E1 )

Sequence Location

Target / Docking site

Mi{MIC}VGlut1^MI04979

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

22E1 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

Mi{Trojan-QF2.2}

(Diao et al., 2015)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

(Diao et al., 2015, Kallman et al., 2015)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ncra\QF^{VGlut1-MI04979-TQF2.2}

Phenotypic Class Phenotype Manifest in Detailed Description

VGlut1^{MI04979-TQF2.2}

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

glutamatergic neuron

(Diao et al., 2015)

embryonic/larval motor neuron

(Diao et al., 2015)

wandering third instar larval stage

glutamatergic neuron

(Venkatasubramanian et al., 2019)

embryonic/larval motor neuron

(Venkatasubramanian et al., 2019)

pupal stage -- adult stage

glutamatergic neuron

(Venkatasubramanian et al., 2019)

(Venkatasubramanian et al., 2019)

leg muscle motor neuron

(Venkatasubramanian et al., 2019)

adult ellipsoid body extrinsic ring neuron

(Xie et al., 2017)

small field neuron of the central complex | restricted

(Xie et al., 2017)

glutamatergic neuron of wing

(Szabó et al., 2023)

(Szabó et al., 2023)

adult female pheromone-sensing neuron of leg

(Kallman et al., 2015)

Additional Information

Statement

Reference

NcraQF^{VGlut-MI04979-TQF2.2} drives expression in neurons that use glutamate as a neurotransmitter, and replicates the VGlut1 immunoreactivity pattern.

(Diao et al., 2015)

Marker for

Reflects expression of

(Szabó et al., 2023, Venkatasubramanian et al., 2019, Xie et al., 2017, Diao et al., 2015, Kallman et al., 2015)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Replacement descendant of (target)

Mi{MIC}VGlut1^MI04979

(Diao et al., 2015)

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

60315

w^*; Mi{Trojan-QF2.2}VGlut1^{MI04979-TQF2.2}/CyO; P{QUAS-mCD8-GFP.P}5B

External Crossreferences and Linkouts ( 0 )

Comments

Comments

phiC31-mediated recombination has been used to replace the attP cassette of the Mi{MIC} element with the intron phase 2 "Trojan QF2" cassette from the pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70 plasmid.

(Diao et al., 2015)

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

DvGlut-T2A-QF2

(Giachello et al., 2022)

Mi{Trojan-QF2.2}VGlut1^{MI04979-TQF2.2}

Mi{Trojan-QF2.2}VGlut^{MI04979-TQF2.2}

TrojanVglut-QF2

(Corty et al., 2022)

VGlut-QF

(Szabó et al., 2023, Venkatasubramanian et al., 2019)

VGlut-QF2

(Lassetter et al., 2023)

VGlut^MI04979-QF2

(Diao et al., 2015)

Vglut-QF2

(Corty et al., 2022)

Vglut-QF2^{MI04979-TQF2.2}

(Lassetter et al., 2023)

Vglut-T2A-QF2

(Venkatasubramanian et al., 2019)

dvGlut^MI04979-QF2

(Wei et al., 2020)

vGluT^MI04979-GAL4

(Xie et al., 2017)

vGlut^MI04979-QF2

(Kallman et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections