A set of transgenic insertion stocks containing a protein trap cassette, derived from Mi{MIC} insertions in coding introns using Recombinase-Mediated Cassette Exchange (RMCE); see http://flypush.imgen.bcm.tmc.edu/pscreen/technique.html. The protein trap cassette contains a EGFP-FlAsH-StrepII-TEV-3xFlag ("GFSTF") tag that is flanked on either side with a 4X(GlyGlySer) flexible linker; the mini-yellow marker is no longer present. Each insertion line contains one of three versions of the cassette (Mi{PT-GFSTF.0}, Mi{PT-GFSTF.1} or Mi{PT-GFSTF.2}), depending on the reading frame required to generate an in-frame fusion protein with the gene in which the insertion is located. This system also allows UAS/GAL4-based knockdown strategies targeting GFP-sequence containing mRNA (RNAi against GFP) or GFP fusion protein (deGradFP).
MI04996-GFSTF.0
The parent MI04996 MiMIC line contains a single insertion, but the flanking sequence matches a duplicated region of the reference genome. Both flanks align equally well at two sites: 3R:9696087 (+) (release 6) in a coding intron of Unc-115a and 3R:9688201 (+) in a coding intron of Unc-115b and intron of 3' UTR of one annotated isoform of p24-2. Thus there are two possible locations for the parent MI04996 MiMIC line, 7.9kb apart and it is not known to which location the RMCE-derived Mi{PT-GFSTF.0}MI04996 line maps, or which of Unc-115a or Unc-115b the Mi{PT-GFSTF.0}MI04996 line is predicted to protein trap.