The homology arms of the donor plasmid used in generating the TI{CRIMIC.TG4.2}dxCR00515-TG4.2-X insertion were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion is predicted to be accompanied by a deletion of 7bp of genomic sequence flanking the insertion site.
CR00515
Not predicted to express GAL4 because the reading frame of the T2A-GAL4 of the CRIMIC donor construct is out of frame with the annotated reading frame of the protein.