FlyBase Insertion Report: Dmel\TI{CRIMIC.TG4.2}Hr38[CR00587-TG4.2]

General Information

Symbol

Dmel\TI{CRIMIC.TG4.2}Hr38^{CR00587-TG4.2}

Species

D. melanogaster

Name

FlyBase ID

FBti0200596

Feature type

insertion_site

Description

Inserted Element

TI{CRIMIC.TG4.2}

LINE ID

CR00587

Affected gene(s)

Hr38

Viability / fertility

Associated allele(s)

Hr38^{CR00587-TG4.2}, Scer\GAL4^{Hr38-CR00587-TG4.2}

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 38E1 )

Sequence Location

2L:20,689,653..20,689,684 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:20,689,653..20,689,684 [-]

(Levis, 2018.8.30)

Orientation

(Levis, 2018.8.30)

Cytological location (computed by FlyBase)

38E1 (inferred by FlyBase from sequence location)

Cytological location (reported)

38E1_r6 (reported as inferred from sequence location)

(Levis, 2018.8.30)

Insertion into Natural transposon

Comments concerning location

The homology arms of the donor plasmid used in generating the TI{CRIMIC.TG4.2}Hr38^{CR00587-TG4.2} insertion were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion is predicted to be accompanied by a deletion of 31bp of genomic sequence flanking the insertion site.

(Levis, 2018.8.30)

Sequence Data

Flanking sequence

Inserted Element

Construct

TI{CRIMIC.TG4.2}

(Lee et al., 2018)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

Hr38

(Lee et al., 2018, Levis, 2018.8.30)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Hr38^{CR00587-TG4.2}

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^{Hr38-CR00587-TG4.2}

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

CR00587

(Lee et al., 2018, Levis, 2018.8.30)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

83181

y¹ w^*; TI{CRIMIC.TG4.2}Hr38^{CR00587-TG4.2}/SM6a

External Crossreferences and Linkouts ( 1 )

Linkouts

GDP-CRIMIC

CR00587

Comments

Not predicted to express GAL4 because the reading frame of the T2A-GAL4 of the CRIMIC donor construct is out of frame with the annotated reading frame of the protein.

(Levis, 2018.8.30)

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

TI{CRIMIC.TG4.2}Hr38^{CR00587-TG4.2}

(Levis, 2018.8.30)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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