The homology arms of the donor vector overlapped by 6 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.
Not predicted to express GAL4 because the reading frame of the T2A-GAL4 of the CRIMIC donor construct is out of frame with the annotated reading frame of the protein.
Not predicted to express GAL4 because the reading frame of the T2A-GAL4 of the CRIMIC donor construct is out of frame with the annotated reading frame of the protein.