FlyBase Insertion Report: Dmel\TI{T-GEM.2}α-Spec[CR00273-TG4.2]

General Information

Symbol

Dmel\TI{T-GEM.2}α-Spec^{CR00273-TG4.2}

Species

D. melanogaster

Name

FlyBase ID

FBti0206940

Feature type

insertion_site

Description

Inserted Element

TI{T-GEM.2}

LINE ID

CR00273

Affected gene(s)

α-Spec

Viability / fertility

Associated allele(s)

Scer\GAL4^{α-Spec-CR00273-TG4.2}, α-Spec^{CR00273-TG4.2}

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 62B4 )

Sequence Location

3L:1,780,601..1,780,625 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:1,780,601..1,780,625 [-]

(Gene Disruption Project members, 2018-)

Orientation

(Gene Disruption Project members, 2018-)

Cytological location (computed by FlyBase)

62B4 (inferred by FlyBase from sequence location)

Cytological location (reported)

62B4_r6 (reported as inferred from sequence location)

(Gene Disruption Project members, 2018-)

Insertion into Natural transposon

Comments concerning location

The homology arms of the donor plasmid used in generating the TI{T-GEM}α-Spec^{CR00273-TG4.2} insertion were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion is predicted to be accompanied by a deletion of 24bp of genomic sequence flanking the insertion site. The PCR check of the insertion gave a shorter than expected band for the right flank and the expected sized product for the left flank.

(Gene Disruption Project members, 2018-)

Sequence Data

Flanking sequence

Inserted Element

Construct

TI{T-GEM.2}

(FlyBase, 1992-)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene