FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\TI{vas-Cas9.T2A.GFP,attP}SGyA
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General Information
Symbol
Dmel\TI{vas-Cas9.T2A.GFP,attP}SGyA
Species
D. melanogaster
Name
FlyBase ID
FBti0213085
Feature type
insertion_site
Description
Inserted Element
TI{vas-Cas9.T2A.GFP,attP}
LINE ID
Affected gene(s)
kl-2
Viability / fertility
Associated allele(s)
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
Y
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)

Y

(Gamez and Akbari, 2020.10.14)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location

The TI{vas-Cas9.T2A.GFP,attP}SGyA insertion maps to the Y chromosome. The Cas9-mediated insertion event targeted a site 3' of the kl-2 gene.

(Gamez and Akbari, 2020.10.14)
Sequence Data
Flanking sequence
Inserted Element
Construct
TI{vas-Cas9.T2A.GFP,attP}
(Gamez and Akbari, 2020.10.14)
Size (Kb)
Component Allele(s)
Avic\GFPEGFP.vas.A
Spyo\Cas9vas.PA
Molecular map
Affected Gene(s)
Insertion may affect gene
kl-2
(Gamez and Akbari, 2020.10.14)
Variant Molecular Consequences
Phenotypic Data
Associated Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Despite the presence of insulator sequences in TI{vas-Cas9.T2A.GFP,attP}, the Disc\RFPtdTom.3xP3 marker is not completely insulated from heterochromatic suppression in the TI{vas-Cas9.T2A.GFP,attP}SGyA insertion; the marker shows variable expression in this insertion line and often appears completely suppressed. Nevertheless, Cas9 expression from this insertion has been shown to be sufficient to allow guide RNA-based cleavage.

(Gamez and Akbari, 2020.10.14)
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
SGyA
(Gamez et al., 2021)
TI{vas-Cas9.T2A.GFP,attP}SGyA
Name Synonyms
Secondary FlyBase IDs
    References (3)