A mutated twi proximal element (PE) in which the core sequence of the low affinity TD5 dl binding site has been mutated to the optimal sequence to create a high affinity site (sequence of the low affinity TD4 site is unchanged), followed by twi sequence from -180 to +160bp relative to the transcription start site, are fused upstream of lacZ.
construct_comment: One of a series of constructs characterizing 5\' regulatory regions of twi, in particular the dl binding sites. Additional related constructs listed under P{twi-lacZ.P}.