A mutated twi proximal element (PE), in which i. the core sequences of the low affinity TD4 and TD5 dl binding sites have been mutated to the optimal sequence to create high affinity sites and ii. two copies of an E box with the core sequence CAGGTG (recognized by both bHLH activators and sna) have been inserted just upstream of the TD4 and TD5 dl binding sites, followed by twi sequence from -180 to +160bp relative to the transcription start site, are fused upstream of lacZ.
Expressed in two lateral stripes that are 4-5 cells wide, similar but not identical to the rho expression pattern. Expression is excluded from the ventral-most 18 cells.
construct_comment: One of a series of constructs characterizing 5\' regulatory regions of twi, in particular the dl binding sites. Additional related constructs listed under P{twi-lacZ.P}.