UAS regulatory sequences drive expression of exd with a deletion of the polyalanine domain that separates the PBC-A and PBC-C domains. In addition, the key amino acid residues of the nuclear export signal (NES) have been mutated to alanine (amino acid replacements I209A, F213A, I216A and M218A). The protein is tagged at the N-terminal end with Tag:MYC and GFP.