FB2026_02 , released June 18, 2026
Recombinant Construct: P{Dad-EGFP.nuclear}
Open Close
General Information
Symbol
P{Dad-EGFP.nuclear}
FlyBase ID
FBtp0055439
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPEGFP.Dad.Tag:NLS(tra)
Also Known As
DadGFP, dad-gfp(nuclear), Dad4-GFP, dad::nlsGFP
Size
Expression Data
GFP reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPEGFP.Dad.Tag:NLS(tra)
Transgenic product class
Component
Use(s)
Regulatory region(s)
Dad
Encoded product / tool
EGFP
Tagged with
Tag:NLS(tra)
Description

A 2250bp fragment comprising the second intron of the Dad-RA transcript, which contains the minimal Dad enhancer (Dad13), drives expression of EGFP tagged with a nuclear localization signal (Tag:NLS(tra)).

(Ninov et al., 2010)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 13 -- 16
embryonic/larval anterior Malpighian tubule | restricted
(Weavers and Skaer, 2013)
Additional Information
Statement
Reference

Expression occurs in the "kink" cells of the anterior Malpighian tubules.

(Weavers and Skaer, 2013)
 
Marker for
Reflects expression of
Dad
(Weavers and Skaer, 2013)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
Reported As
Symbol Synonym
Dad4-GFP
(Bieli et al., 2015)
Dad::n::GFP
(Djabrayan and Casanova, 2016)
DadGFP
(Weavers and Skaer, 2013, Bunt et al., 2010)
P{Dad-EGFP.nuclear}
(FlyBase, 1992-)
P{Dad4::EGFPnuc, w+}
(Bieli et al., 2015)
dad-EGFPnuclear
(Ninov et al., 2010)
dad-gfp
(Weavers and Skaer, 2013)
dad-gfp(nuclear)
(Weavers and Skaer, 2013, Bunt et al., 2010)
dad4-GFP
(Vuilleumier et al., 2010)
dad::nlsGFP
(Ayyaz et al., 2015, Li et al., 2013)
Secondary FlyBase IDs
    References (10)